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Clinical Chemistry

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Clinical chemistry (also known as chemical pathology, clinical biochemistry or medical biochemistry) is the area of chemistry that is generally concerned with analysis of bodily fluids for diagnostic and therapeutic purposes. It is an applied form of biochemistry (not to be confused with medicinal chemistry, which involves basic research for drug development).
The discipline originated in the late 19th century with the use of simple chemical reaction tests for various components of blood and urine. In the many decades since, other techniques have been applied as science and technology have advanced, including the use and measurement of enzyme activities, spectrophotometry, electrophoresis, and immunoassay. There are now many blood tests and clinical urine tests with extensive diagnostic capabilities.
Most current laboratories are now highly automated to accommodate the high workload typical of a hospital laboratory. Tests performed are closely monitored and quality controlled.
All biochemical tests come under chemical pathology. These are performed on any kind of body fluid, but mostly on serum or plasma. Serum is the yellow watery part of blood that is left after blood has been allowed to clot and all blood cells have been removed. This is most easily done by centrifugation, which packs the denser blood cells and platelets to the bottom of the centrifuge tube, leaving the liquid serum fraction resting above the packed cells. This initial step before analysis has recently been included in instruments that operate on the “integrated system” principle. Plasma is in essence the same as serum, but is obtained by centrifuging the blood without clotting. Plasma is obtained by centrifugation before clotting occurs. The type of test required dictates what type of sample is used.
A large medical laboratory will accept samples for up to about 700 different kinds of tests. Even the largest of laboratories rarely do all these tests themselves, and some must be referred to other labs.
This large array of tests can be categorised into sub-specialities of:
• General or routine chemistry – commonly ordered blood chemistries (e.g., liver and kidney function tests).
• Special chemistry – elaborate techniques such as electrophoresis, and manual testing methods.
• Clinical endocrinology – the study of hormones, and diagnosis of endocrine disorders.
• Toxicology – the study of drugs of abuse and other chemicals.
• Therapeutic Drug Monitoring – measurement of therapeutic medication levels to optimize dosage.
• Urinalysis – chemical analysis of urine for a wide array of diseases, along with other fluids such as CSF and effusions
• Fecal analysis – mostly for detection of gastrointestinal disorders.


Point-of-care

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Point-of-care testing (POCT), or bedside testing is defined as medical testing at or near the site of patient care.[1] These are simple medical blood tests which can be performed at the bedside. Simple tests such as those found in medical examinations, urine test strips and even simple imaging such as with a portable ultrasound device. As well as regular observations such as ECG’s, O2 saturation and heart rate. A recent survey in five countries (Australia, Belgium, the Netherlands, the UK and the US) indicates that general practitioners / family doctors would like to use more POCTs.


Immunofluorescence

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Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualisation of the distribution of the target molecule through the sample. Immunofluorescence is a widely used example of immunostaining and is a specific example of immunohistochemistry that makes use of fluorophores to visualise the location of the antibodies.Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyse the distribution of proteins, glycans, and small biological and non-biological molecules.


Cytology

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The medical and scientific study of cells. Cytology refers to a branch of pathology, the medical specialty that deals with making diagnoses of diseases and conditions through the examination of tissue samples from the body.Cytologic examinations may be performed on body fluids (examples are blood, urine, and cerebrospinal fluid) or on material that is aspirated (drawn out via suction into a syringe) from the body. Cytology also can involve examinations of preparations that are scraped or washed (irrigated with a sterile solution) from specific areas of the body.


Coagulation

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Coagulation (also known as clotting) is the process by which blood changes from a liquid to a gel. It potentially results in hemostasis, the cessation of blood loss from a damaged vessel, followed by repair. The mechanism of coagulation involves activation, adhesion, and aggregation of platelets along with deposition and maturation of fibrin. Disorders of coagulation are disease states which can result in bleeding (hemorrhage or bruising) or obstructive clotting (thrombosis).Coagulation is highly conserved throughout biology; in all mammals, coagulation involves both a cellular (platelet) and a protein (coagulation factor) component.The system in humans has been the most extensively researched and is the best understood.


Elisa

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ELISA Technologies, Inc. was founded in 1991 to develop enzyme immunoassay technology for use in the food industry. Our mission is to assure food quality by creating and supporting rapid, reliable, sensitive and cost-effective testing solutions. We are committed to using our expertise in species, allergens, antibiotics, drugs, hormones, toxins and other analytes to assist our clients with their analytic needs. Our commitment is to provide our clients with accurate, confidential, efficient, reliable and cost-effective analytical options to meet their needs.


Microbiology

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Microbiology (from Greek μῑκρος, mīkros, “small”; βίος, bios, “life”; and -λογία, -logia) is the study of microscopic organisms, either unicellular (single cell), multicellular (cell colony), or acellular (lacking cells).Microbiology encompasses numerous sub-disciplines including virology, mycology, parasitology, and bacteriology.

Did you know that?

Scientists estimate that laughing 100 times is equivalent to a 10-minute workout on a rowing machine.


Immunology

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At Green Cross laboratory, we focus on bridging the transparency gap. We build a structure of ethical systems, as we understand that getting the right culture means reasserting a simple truth: every sample or test is a real person. We genetically code care, compassion and dignity in our work culture and take immense pride in being recognized for the accuracy and reliability of our results. We recognize how important the results are to you and to your loved ones and so we make it out utmost important duty to give you a correct and reliable final outcome of the tests.

We consider it our pleasure to serve people and take care of their needs, we constantly innovate to find new ways to keep improving our services.

Green Cross Laboratory is more about names than numbers and even more about the feelings related to the names we write on the test results.

Did you know that?

Scientists estimate that laughing 100 times is equivalent to a 10-minute workout on a rowing machine.


Immunohistochemistry

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Biochemistry, sometimes called biological chemistry, is the study of chemical processes within and relating to living organisms. By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism, biochemical processes give rise to the complexity of life. Over the last 40 years, biochemistry has become so successful at explaining living processes that now almost all areas of the life sciences from botany to medicine are engaged in biochemical research.Today, the main focus of pure biochemistry is in understanding how biological molecules give rise to the processes that occur within living cells, which in turn relates greatly to the study and understanding of whole organisms.

Did you know that?

Scientists estimate that laughing 100 times is equivalent to a 10-minute workout on a rowing machine.


Histopathology

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Histopathology (compound of three Greek words: ἱστός histos “tissue”, πάθος pathos “suffering”, and -λογία -logia “study of”) refers to the microscopic examination of tissue in order to study the manifestations of disease. Specifically, in clinical medicine, histopathology refers to the examination of a biopsy or surgical specimen by a pathologist, after the specimen has been processed and histological sections have been placed onto glass slides. In contrast, cytopathology examines free cells or tissue fragments.

Did you know that?

Quick naps not only improve your alertness, but they also help in decision making, creativity and sensory perception.


Hematology

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Hematology, also spelled haematology or hæmatology (from the Greek αἷμα, haima “blood” and -λoγία), is the branch of medicine concerned with the study, diagnosis, treatment, and prevention of diseases related to the blood. Hematology includes the study of etiology.It involves treating diseases that affect the production of blood and its components, such as blood cells, hemoglobin, blood proteins, and the mechanism of coagulation. The laboratory work that goes into the study of blood is frequently performed by a medical technologist. Hematologists also conduct studies in oncology—the medical treatment of cancer.

Did you know that?

An average heart beats 100,000 times a day, pumping some 2,000 gallons of blood through its chambers. Over a 70-year life span, that adds up to more than 2.5 billion heartbeats.


Health Check Up

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Whole Body Check (AWBC)
This includes a Comprehensive check of all body systems including Cardiac status,and Biochemical profile (including Blood sugars,Lipid profile,Eye checkup,ENT and Dental checkup ,and Mammography for Women)It gives a detailed assessment of the individual and is strongly recommended for all individuals above 40 years.
    • Haemogram  – Hemoglobin, PVC, RBC, MCHC, MCV, MCH, Total WBC/Differential Count, ESR, Platelet count.
    • Biochemical Parameters  – FBS,PPPBS,HbA1c, Blood Urea, S. Creatinine, S. Uric Acid
    • Liver Function Test  – Total Protein, Albumin, Globulin, A/G ratio, SGOT, SGPT, Alkaline Phosphatase, Bilirubin ( Total & Direct ), Gamma GT
    • Lipid Profile  – Total Cholesterol, HDL Cholesterol, LDL Cholesterol, Triglycerides, Total Cholesterol/ HDL Ratio.
    • General Tests  – Blood grouping and RH Typing, Urine Routine, Stool routine test, Resting ECG, X-ray-Chest, Ultrasonogram of the Abdomen (Screening only) and Pap smear (for Women)
    • Cardiac Stress Analysis(TMT/CSA)
    • Pulmonary Function test
    • Echocardiography
    • S. Calcium and Phosphorous
    • S. Electrolytes
    • HbsAg
    • Mammogram for Women

Molecular Diagnostic

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High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytic chemistry used to separate the components in a mixture, to identify each component, and to quantify each component. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

HPLC has been used for medical (e.g. detecting vitamin D levels in blood serum), legal (e.g. detecting performance enhancement drugs in urine), research (e.g. separating the components of a complex biological sample, or of similar synthetic chemicals from each other), and manufacturing (e.g. during the production process of pharmaceutical and biological products) purposes.